Then, the effect of 7HF treatment (6, 12, and 24 ВµM) on the dynamic arrangement of MTs was assessed for 1, 12, and 24 h using indirect immunofluorescence. The drug-like properties of 7HF were predicted using the Swiss-absorption, distribution, metabolism, and excretion (ADME) webtool. MethodsД§HF uptake by MDA-MB-468 cells was assessed using spectrophotometry. Here, the activity and mechanism via which 7HF controls MTs dynamics was investigated in MDA-MB-468 cells. However, antimitotic drug cytotoxicity to multiple cancer resistance developed in response to drugs are obstacles faced to date. Microtubules (MTs) dynamically regulate cell division in the G2/M phase and are related to cancer cell stress response. 7-О±-Hydroxyfrullanolide (7HF) possesses anticancer properties and arrests cells in the G2/M-phase via modulation of several proteins involved in the G2/M-phase transition, as well as the mitotic checkpoint in MDA-MB-468 (TNBC) cells. Triple-negative breast cancer (TNBC) responds poorly to the available drugs thus, the mortality rate associated with TNBC remains high. Anticancer mechanism of 7-О±-hydroxyfrullanolide on microtubules and computational prediction of its target binding in triple-negative breast cancer cells. Cite this article Chimplee S, Smythe C, Tipmanee V, Sukrong S, Kanokwiroon K. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Licence This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. Set the transparent character(s) for the top layer (Space by default).3 Center of Excellence in DNA Barcoding of Thai Medicinal Plants, Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand DOI 10.7717/peerj.13508 Published Accepted Received Academic Editor Vladimir Uversky Subject Areas Biochemistry, Cell Biology, Computational Biology, Molecular Biology Keywords 7-О±-Hydroxyfrullanolide, Sesquiterpene lactones, Natural product, Triple-negative breast cancer, Microtubules, G2/M arrest, G1 arrest, DNA damage response, Molecular docking Copyright В© 2022 Chimplee et al. Merge two text files вЂ“ Set the two input fields below the canvas, shift them to place them on top of each other.Set the dimensions of the image and the used characters (grey ramp: from dark to light) It works best for high contrast pictures. Image converter вЂ“ Convert your images to ASCII art with this option.Make sure you move your mouse slowly to avoid ruptures. Draw line вЂ“ Draw a continious double or single line with your mouse.Find and replace вЂ“ Substitutes every occurence of a string with another one.Character picker вЂ“ Pick a field on the canvas and the program will set the selected character in the palette.Type a multilne text in the input to add it to a selected position on the canvas with the Insert option. When the selection is done hit the Delete, Crop or Copy options. A text area will show the current selection in real time. Select / Highlight вЂ“ Select a rectangle area on the paintboard to highlight it.Use the arrow buttons to change the cursor position. Type text вЂ“ Click a field on the canvas and start typing with your keyboard.Paint bucket / Fill area вЂ“ Pick a character just like for the previous Pencil option and click an isolated area on the canvas to fill it with the selected letter or symbol.Search characters by name to add them to the palette. Picking a character in the palette is the quickest way of changing the active character with a single click. Picking the 'Input' option can be edited as an input field. The delete/erase option simply fills the cells with Space (empty) characters. Pencil / Draw вЂ“ Select the brush size from 1x1px to 4x4 and pick a character to draw with on the canvas.Use it like the classic Paint program, but instead of pixels and colors add characters to the canvas.ДЇind the available painting options in the left sidebar and the canvas on the right. рџ…Ѕрџ…ґрџ†ѓ - Draw and Paint Text on the CanvasДraw text with ASCII and unicode characters with your mouse on the canvas.

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